ABSTRACT. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Plant Adhatoda vasica L. Nees (Acanthaceae) commonly known as Malabar nut is an evergreen two – three m tall shrub, sometime used as. Adhatoda vasica (L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the .

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Evaluation of antioxidant activity of isoferulic anrimicrobial in vitro. Two mobile phases A and B were used at flow rate of 1. Results and Discussion The ethanol extract of A. Antimicrobial and antimycobacterial activities of methyl caffeate isolated from Solanum torvum Swartz.

Antibacterial Activity of Leaf Extracts of Adhatoda vasica – MAHE Digital Repository

Vasicine acetate showed minimum inhibitory concentration values against bacteria; they were M. Natural products still remain the most important source for discovery of new and potential drug molecules. The emergence of drug-resistant pathogens and the increase in diseases affecting the immune system have greatly intensified the need to investigate new bioactive metabolites for potential pharmaceutical and industrial applications [ 12 ].

Vasicine showed antimicrobial activity against S. The MIC for fungi was defined antimicrobiall the lowest extract concentration, showing no visible fungal growth after incubation time. Antimicrobial Activity Antibacterial and antifungal activities were carried out using disc diffusion method [ 16 ].


Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Accumulation of vasicine and vasicinone in tissue cultures of Adhatoda vasica and evaluation of the free radical-scavenging activities of the various crude extracts. Mobile phase A consisted of water with 0. Shade dried and coarsely powdered leaves of A. After various durations of cultivation, the solution in the medium was removed.

Antibacterial activity of adhatoda vasica leaf extract

Journal List Biomed Res Int v. Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis Lep. Other constituents include vitamin C, saponins, flavonoids as well as steroids, antimicrobiial fatty acids [ 13 ].

Cytotoxicity of each sample was expressed as IC 50 value. International Journal of Cancer. Kulkarni [ 24 ] reported that A. Zones of inhibition were recorded in millimetres and the experiment was repeated twice.

Nature acts as a prominent reservoir for new and novel therapeutics. HPLC analysis was carried out on a Waters Alliance separations module with photodiode array detector Waters, Sample ethanol extract Antitussive effect aduatoda Adhatoda vasica extract on mechanical or chemical stimulation-induced coughing in animals. Indian Journal of Natural Products and Resources. UV-Visible spectra were taken in methanol on Thermo Fisher instrument.

Vasicine acetate was obtained by acetylation of Vasicine. Sankar2 and K. National Center for Biotechnology InformationU. The plant is reported to show abortifacient [ 7 ], antimicrobial [ 89 ], and antitussive activities [ adhattoda ].


Then the residue was crystallised from chloroform to get Vasicine acetate as colorless crystals m.

Effect of Adhatoda vasica massage in pyorrhoea. Medicinal plants are important sources of practical drugs for people throughout the year. The fraction was further purified and the compound Vasicine was isolated.

BHT is used as standard of antioxidant. Classical Uses of Medicinal Plants. The mobile phase was filtered through a 0. Vasicine acetate exhibited good zone of inhibition against bacteria: Indian Journal of Microbiology. Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper. Antimlcrobial chemicals are given in delta scale with TMS as an internal standard.

Streptomycin was used as positive control for bacteria and Ketoconazole as positive control for fungi.

All melting point values are uncorrected and taken by open capillary method on a heating block Instrument. Glossary of Indian Medicinal Plants. The worldwide threat of antimicrobial resistance. The crude extract of A.